Articles

Enhancing in vitro osteogenic differentiation of mesenchymal stem cells via sustained dexamethasone delivery in 3D-Printed hybrid scaffolds based on polycaprolactone-nanohydroxyapatite/alginate-gelatin for bone regeneration

Layer-by-layer 3D-printing was effectively applied to prepare a biomimetic combined structure composing hard composite (PCL-nHA) and soft hydrogel (Alg-Gel).

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Dual-responsive synthetic gene circuit for dynamic biologic drug delivery via inflammatory and circadian signaling pathways

Engineered cells provide versatile tools for precise, tunable drug delivery, especially when synthetic stimulus-responsive gene circuits are incorporated. In many complex disease conditions, endogenous patholo...

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Core-shell nanofiber dressings with zinc oxide nanoparticles and cell-free fat extract: boosting fibroblast activity and antibacterial efficacy

This study presents the development and characterization of innovative core-shell nanofiber wound dressings incorporating zinc oxide nanoparticles (nZnO) and cell-free fat extract (CEFFE) to enhance fibroblast...

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Design of a prodrug bispecific antibody masked by a functional molecule for lymphocyte activation for cancer therapy

Although T cells engaging bispecific antibodies (T-bsAbs) have shown great benefits, their use in treating solid tumors is challenging because of the minimal infiltration of T-cells. We fused an agonistic sing...

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From microbial proteins to cultivated meat for alternative meat-like products: a review on sustainable fermentation approaches

The global demand for protein is rapidly increasing due to population growth and changing dietary preferences, highlighting the need for sustainable alternatives to traditional animal-based proteins. This revi...

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Effects of antifoam agents on Spodoptera frugiperda 9 cell growth and baculovirus infection dynamics

The baculovirus expression system is widely used for recombinant protein production. However, its scalability under shaking or stirring cultivation conditions remains a challenge due to foam formation which ca...

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Phenolic-loaded nanofiber from Arctium lappa root: a potential therapy for testosterone-induced ovarian oxidative stress

Polycystic Ovary Syndrome (PCOS) is a hormonal disorder affecting women of reproductive age, often associated with oxidative stress and inflammation. This study explores the therapeutic potential of Arctium lappa

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High-throughput strategies for monoclonal antibody screening: advances and challenges

Antibodies characterized by high affinity and specificity, developed through high-throughput screening and rapid preparation, are crucial to contemporary biomedical industry. Traditional antibody preparation v...

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Engineered supercooling systems for enhanced long-term preservation of large-volume red blood cells in commercial blood bags

Reducing cell metabolism by lowering the storage temperature is an important method to improve the quality of stored RBCs and prolong the stored shelf life of RBCs. Traditional cryopreservation suffers from li...

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Cerium-doped calcium carbonate microparticles combined with low-intensity ultrasound for efficient sonodynamic therapy in body sculpting

Excessive caloric intake and reduced energy expenditure contribute to obesity and localized fat accumulation, adversely affecting overall health. Despite advancements in obesity treatments, noninvasive approac...

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Decellularized apple hypanthium as a plant-based biomaterial for cartilage regeneration in vitro: a comparative study of progenitor cell types and environmental conditions

Decellularized plant tissues have been shown to enhance the integration and proliferation of human cells, demonstrating biocompatibility. These tissues are now being considered as valuable biomaterials for tis...

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Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management

A light-producing secretory protein that is collectible through the supernatant of a culture medium is essential in a cell-based reporter gene system and allows for real-time monitoring of upstream events of a...

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Anti-inflammatory activity of magnetic fields emitted by graphene devices on cultured human cells

Inflammation plays a key role in various diseases such as pancreatitis, cancer, and rheumatoid arthritis. Acute inflammation involves processes like vasodilation, increased vascular permeability, and leukocyte...

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Engineering FcRn binding kinetics dramatically extends antibody serum half-life and enhances therapeutic potential

Optimizing the IgG Fc domain for neonatal Fc receptor (FcRn) binding is crucial for enhancing antibody pharmacokinetics. The prolonged serum half-life of IgG antibody is governed by its pH-dependent interactio...

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Unraveling the unique bioactivities of highly purified C-phycocyanin and allophycocyanin

The blue-green microalgae Spirulina, used in human nutrition for centuries, includes phycobiliproteins such as C-phycocyanin (CPC) and allophycocyanin (APC). Assessing their unique bioactivities separately is ...

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Correction: Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures

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Unsilencing a cryptic xylose metabolic pathway in Rhodococcus jostii RHA1 for efficient lipid production from lignocellulosic biomass

Rhodococcus jostii RHA1 is an oleaginous bacterium that has attracted considerable attention due to its capacity to use different carbon sources to accumulate significant levels of triacylglycerols that might be ...

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Biomaterial-based chitosan nanohydrogel films: combination of Bistorta officinalis and Ca-doped carbon dots for improved blood clotting

Bleeding and traumatic injuries are still a major issue necessitating the development of advanced hemostatic materials that are economical, biocompatible, and effective. Chitosan’s (CS) haemostatic and biocomp...

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Development of a CRISPR-based cytosine base editor for restriction-modification system inactivation to enhance transformation efficiency in Vibrio Sp. dhg

Vibrio sp. dhg is a fast-growing, alginate-utilizing, marine bacterium being developed as a platform host for macroalgae biorefinery. To maximize its potential in the production of various value-added products, t...

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Termination sequence between an inducible promoter and ubiquitous chromatin opening element (UCOE) reduces gene expression leakage and silencing

Inducible gene expression circuits enable precise control over target gene activation and are widely used in direct reprogramming. However, their usability is often compromised by DNA methylation-induced silen...

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Engineering the T6SS of Pseudomonas for targeted delivery of antibacterial and antifungal effectors

Bacteria employ diverse molecular systems, such as the type VI secretion system (T6SS) to outcompete other microorganisms and adapt to ecological niches. The T6SS is a versatile nanomachine capable of deliveri...

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Manipulating subcellular protein localization to enhance target protein accumulation in minicells

Minicells are chromosome-free derivatives of bacteria formed through irregular cell division. Unlike simplified structures, minicells retain all cellular components of the parent cell except for the chromosome...

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CRISPRi-assisted metabolic engineering of cyanobacteria for photosynthetic hyaluronic acid from CO₂

Hyaluronic acid (HA) is widely used in pharmaceuticals, medicine, and cosmetics. Sustainable production has shifted to microbial fermentation using engineered GRAS strains. Diverse carbon sources and CO₂ conversi...

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Gene syntaxes modulate gene expression and circuit behavior on plasmids

Achieving consistent and predictable gene expression from plasmids remains challenging. While much attention has focused on intra-genetic elements like promoters and ribosomal binding sites, the spatial arrang...

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An electrochemical biosensor for the detection of microRNA-31 as a potential oral cancer biomarker

Oral cancer presents substantial challenges to global health due to its elevated mortality rates. Approximately 90% of these malignancies are oral squamous cell carcinoma (OSCC). A significant contributor to t...

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Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow

Adipose-derived stem cells (ASCs) provide an ample, easily accessible source of multipotent cells, an alternative to bone marrow-derived stromal cells (BMSCs), capable of differentiating into osteoblasts. Howe...

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Correction: Treatment of effluents from Food services establishment (FSEs) by physico-chemical processes: a case study for Trinidad & Tobago

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CRISPR/Cas12a-mediated gene silencing across diverse functional genes demonstrates single gene-specific spacer efficacy in Mycobacterium smegmatis

Tuberculosis, a persistent global health threat, necessitates a comprehensive understanding of the genes and pathways crucial for the survival and virulence of the causative pathogen, Mycobacterium tuberculosis. ...

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Carbon fluxes rewiring in engineered E. coli via reverse tricarboxylic acid cycle pathway under chemolithotrophic condition

A transgenic strain of Escherichia coli has been engineered to directly assimilate gaseous CO₂ into its biomass through hydrogen-powered anaerobic respiration. This was achieved by expressing key components of th...

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Metabolic engineering of Komagataella phaffii for enhanced 3-hydroxypropionic acid (3-HP) production from methanol

Bioconversion of methanol derived from CO₂ reduction into value-added chemicals provides a unique approach for mitigating global warming and reducing fossil fuels dependence. Production of 3-hydroxypropionic acid...

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Bioelectric profiling of Rickettsia montanensis in Vero cells utilizing dielectrophoresis

Rickettsia is an intracellular bacteria transmitted to humans through ticks, lice, fleas, or their feces, causing acute symptoms such as fever, headache, rashes, and muscle aches. Detecting rickettsial disease...

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Reversible fluorescent solid porous films for detection of zinc ions in biological media

The need for a sensitive, selective, non-invasive and reversible fluorescent sensor for Zn²⁺ monitoring is addressed in this work. A novel guest-host system is developed, including a Zn²⁺ sensitive fluorescent pr...

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The stability and self-assembly of tri-calcium silicate and hydroxyapatite scaffolds in bone tissue engineering applications

The fabrication of scaffolds for bone tissue engineering (BTE) applications often involves the utilization of two distinct categories of biomaterials, namely calcium phosphates and calcium silicates. The selec...

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Fabrication of zein nanofibrous scaffold containing Scrophularia striata extract for biomedical application

Skin wounds have the potential to rapidly become infected, with bacteria having the ability to quickly penetrate to the skin’s deeper layers. Then they enter the lymph nodes and spread throughout the body; the...

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Modular plasmid design for autonomous multi-protein expression in Escherichia coli

Molecular and synthetic biology tools enable the design of new-to-nature biological systems, including genetically engineered microorganisms, recombinant proteins, and novel metabolic pathways. These tools sim...

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Nanozyme-based aptasensors for the detection of tumor biomarkers

A nanozyme-based aptasensor combines the unique properties of nanozymes with the specificity of aptamers for the detection of various biomolecules. Nanozymes are nanomaterials that possess enzyme-like properti...

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NIR-activated catechol-functionalized nanodiamond nanofibers for accelerating on-demand MRSA and E. coli biofilm eradication

The rise of antibiotic resistance has made bacterial infections a persistent global health issue. In particular, extracellular polymeric substances (EPS) secreted by bacteria limit the effectiveness of convent...

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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activity

Angiotensin-Converting Enzyme 2 (ACE2) is a crucial peptidase in human peptide hormone signaling, catalyzing the conversion of Angiotensin-II to Angiotensin-(1–7), which activates the Mas receptor and elicits ...

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A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy

Shake flasks are essential tools in biotechnological development due to their cost efficiency and ease of use. However, a significant challenge is the miniaturization of process analytical tools to maximize in...

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Engineering probiotic Escherichia coli for inflammation-responsive indoleacetic acid production using RiboJ-enhanced genetic circuits

As our understanding of gut microbiota’s metabolic impacts on health grows, the interest in engineered probiotics has intensified. This study aimed to engineer the probiotic Escherichia coli Nissle 1917 (EcN) to ...

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Codeine 3-O-demethylase catalyzed biotransformation of morphinan alkaloids in Escherichia coli: site directed mutagenesis of terminal residues improves enzyme expression, stability and biotransformation yield

The cultivation of opium poppy is the only commercially viable source of most morphinan alkaloids. Bioproduction of morphinan alkaloids in recombinant whole-cell systems provides a promising alternate source o...

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Bioprocess exploitation of microaerobic auto-induction using the example of rhamnolipid biosynthesis in Pseudomonas putida KT2440

In biomanufacturing of surface-active agents, such as rhamnolipids, excessive foaming is a significant obstacle for the development of high-performing bioprocesses. The exploitation of the inherent tolerance of P...

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BCL-2 overexpression exosomes promote the proliferation and migration of mesenchymal stem cells in hypoxic environment for skin injury in rats

The direction of this study was to detect and analyze the specific mechanism of anti-apoptosis in mesenchymal stem cells (MSCs) cells caused by high expression of BCL2.

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Engineering Yarrowia lipolytica for the production of β-carotene by carbon and redox rebalancing

β-Carotene is a natural product that has garnered significant commercial interest. Considerable efforts have been made to meet such demand through the metabolic engineering of microorganisms, yet there is stil...

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Scale-up of CHO cell cultures: from 96-well-microtiter plates to stirred tank reactors across three orders of magnitude

For process development in mammalian cell cultivations, scale-up approaches are essential. A lot of studies concern the scale transfer between different-sized stirred tank reactors. However, process developmen...

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Stabilizing milk-derived extracellular vesicles (mEVs) through lyophilization: a novel trehalose and tryptophan formulation for maintaining structure and Bioactivity during long-term storage

Extracellular vesicles (EVs) are widely investigated for their implications in cell-cell signaling, immune modulation, disease pathogenesis, cancer, regenerative medicine, and as a potential drug delivery vect...

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Lithium-doped calcium silicate cement regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration

Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-do...

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Synthesizing hybrid copper phosphate (Cu₃(PO₄)₂) nanoflowers using Cu⁺² and shed snakeskin: antioxidant, antibacterial, anticancer, guaiacol, anionic, and cationic dye degradation properties

Synthesis of organic@inorganic hNFs is achieved by the coordination of organic compounds containing amine, amide, and diol groups with bivalent metals. The use of bio-extracts containing these functional group...

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Advanced metabolic Engineering strategies for the sustainable production of free fatty acids and their derivatives using yeast

The biological production of lipids presents a sustainable method for generating fuels and chemicals. Recognized as safe and enhanced by advanced synthetic biology and metabolic engineering tools, yeasts are b...

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