Fig. 4

REase inactivation on transformation efficiency. Selective plate result showing the transformation of the pACYCDuet™-1 empty plasmid into (A) wt and (B) RE3. (C) Transformation efficiency of wt, RE1, RE2, RE3 shown as the total number of colony forming units (CFU) per µg plasmid as CFU/µg. Data results were calculated as the mean of three independent biological replicates (n = 3) with error bars as ± standard deviation. Statistical significance was calculated based on two-tailed Student’s t-test (*p ≤ 0.05). Table below shows the relative fold increase in transformation efficiency compared to the wt (\(\:\frac{CFU/\mu g\:of\:strain}{CFU/\mu g\:of\:wt}\))