Fig. 3

Multiplex base editing for simultaneous inactivation of REases in the RM system. (A) Schematic of inactivating the REases by premature stop codon formation via multiplex base editing to improve transformation efficiency - Disintegration of foreign DNA by the presence of native REases (left), Inactivation of multiple REases by the CBE system (middle), Enhancement of transformation efficiency due to the absence of active REases (right). (B) Multiplex base editing efficiency of RE1 targets in terms of the ratio of edited genes in each colony (green). (C) Multiplex base editing efficiency of RE2 targets in terms of the ratio of edited genes in each colony (blue)