Fig. 7

Generation of primary human bone spheroids. A-D Visualisation of the method for osteoblast isolation from human bone fragments. E-G Spheroid formation using the PDMS microwell platform cultivated for 3 days. H Analysis of spheroid diameters at different initial seeding densities during 3 days of cultivation (n  = 150). I Actin-normalized mRNA expression levels of osteogenic markers in the monolayer and spheroids were measured by qPCR. n  ≥ 3, * p  = 0.0145, **** p  < 0.0001. Microwell spheroids were captured under 100x magnification